Part:BBa_K1211001:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1211001

This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum. The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA. This mutated sequence has four point mutations and one amino acid substitution (compared to the wild type enzyme) in order to produce (D)-Lactyl-CoA.

We (Yale iGEM 2013) used this enzyme as a the first enzyme in the pathway to create PLA or Polylactic acid. However, this biobrick produces (D)-Lactyl-CoA, which is the precursor to all PHAs, and thus this biobrick can be used in a wide range of projects in the future.

User Reviews

UNIQd90bc43c7a7a916a-partinfo-00000000-QINU UNIQd90bc43c7a7a916a-partinfo-00000001-QINU The Stanford-Brown iGEM team attempted to use this part to help make P(3HB-co-3HV). However, after significant trouble with cloning and sequencing, we came to believe that the sequence submitted to the Registry is quite inconsistent with the sequence uploaded to this Wiki. Our sequencing results of the plasmid obtained from the Registry, without any cloning or modifications, confirmed this. If future teams want to use this part, they should have it synthesized!